(D) BmN cells and BmNPV-infected cells were analyzed by western blot with the indicated antibodies.
For qRT-PCR analysis, BmN cells and BmNPV-infected cells at indicated time were harvested and total RNA was extracted by using TRIzol reagent (Invitrogen). 5μg total RNA was used to synthesis the first-strand cDNAs by TransScript One-Step gDNA Removal and cDNA Synthesis SuperMix (TransGen). qRT-PCR was performed using Hieff qPCR SYBR Green Master Mix (Yeasen) and the primers of qRT-PCR were used as follows: Tua2-F: 5’-TGCCCGAGGACACTATACCA-3’, Tua2-R: 5’- ACACGAGGAAACCCTGAAGC-3’, asf1-F: 5’-CGCCAATGGAATGCTGAGATTAT-3’, asf1-R: 5’-GTACAAACAAAGCCTTGAAAACGG-3’, cbx5-F: 5’-ATCCATTGGAAGGGCTGGTC-3’, cbx5-R: 5’-GGCGCTGCTCACTTTATCCA-3’.
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In contrast, the enrichments of H3K27ac in clusters III and IV sites were markedly higher in BmNPV infected cells than in uninfected cells (Fig 7A, 7D and 7E).
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BmNPV infection results in significant host chromatin marginalization, which has also been found in most DNA viruses.
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Shaded regions represent increase during BmNPV infection.
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The T3 strain of BmNPV was used as wild type (WT) virus in this study.
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(A) Distribution of genomic features of opened or closed peaks during BmNPV infection.
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In this study, we performed ATAC-seq approach to investigate the BmNPV infection mediated changes in genome organization and accessibility.
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Coalition representatives said an infected woman in 2009, from Nevada, may have had an infected boyfriend, and offered evidence that a man infected in 2010 in Florida had worked outside the industry as a prostitute.
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(B) HHV-6 gB expression on mock-infected or HHV-6A-infected HSB-2 cells was determined by immunofluorescence analysis.
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However, when a large proportion of infected individuals are symptomatic, the total number of infected individuals tends to be overestimated, causing large errors in forecasts (cf. the boxplots corresponding to a single infected individual at the time of estimation in Fig 4B).
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A study in macaques infected with the new coronavirus suggested that once infected, the monkeys produce neutralizing antibodies and resist further infection.
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In all years the number of men infected is greater than women (1 mark).
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Instead of focusing on a single infected individual, officials target clusters of infected people.
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C, Representative one-second ECG trace from infected mouse.
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This means that scientists can test the DNA of the infected plant to see if it has been infected by Agrobacterium tumefaciens and confirm that the disease is crown gall disease.
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Two thirds of Denmark’s pig farms are currently infected with CC398, where it is spreading rapidly: 648 people were infected with CC398 in 2013; in 2014, 1,271 people contracted the bug.
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The report concluded that of the 125,000 passengers who flew with the infected passengers, only one additional passenger and two crew members were suspected of being infected due to in-flight transmission.
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Evaluating risk meant trying to cope with unknowns piled on unknowns: the risk of an infected research scientist or wildlife worker encountering bats; the risk of the bats becoming infected in that situation; the risk of an infected bat passing the virus onto other bats so that the virus becomes established in the population.
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While control orders in cases of infectious disease may be justified, there is no requirement for a person to actually be infected or for the officer to even reasonably believe or suspect that the person is infected, or may be infected, with a LHD, before a control order can be made.
BmNPV-infected
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